For fat extraction it was used 10 m

For fat extraction it was used 10 ml of goat milk. Fatty acid methyl esters were prepared by saponification using KOH (0.68M in methanol) followed by transestrification with 20%
boron trifluoride in methanol and analysis by HPLC. The column (900 x 6.4 mm) was Bondapack C-18 and was eluted with acetonitrilewater, at a flow rate of 2ml/min.
The mobile phase was methanol containing some bonds in the same manner and it seems that separations of fatty acid derivatives are less affected by the nature of the gradient when this is used.