The present study has been taken up to establish a suitable protocol for rapid proliferation and in vitro regeneration of Psorelea corylifolia L.and deals with complete regeneration of Psorelea corylifolia. The study indicates that shoot regeneration is the result of interaction between Auxin &Cytokinin in various concentrations and combinations in the medium, while rhizogenesis in regenerated shoot can be obtained by Auxin alone in the medium. Similar effect was also observed by some workers in recent studies21.The present study is a maiden attempt to assess the regenerative capacity of the explants under different hormonal conditions. The method used in the study is reproducible and can be applied for germplasm conservation & large scale regeneration of important medicinal plants.