 Plugs can be lysed for longer per

 Plugs can be lysed for longer periods of time (3-16 hours).
 The washing steps with TE to remove the lysis buffer from the PFGE plugs can be done for longer periods of time
(30-45 min) and at lower temperatures (37°C or room temperature). They can be started on Day 1 and finished on
Day 2 after overnight refrigeration of the plugs in TE.