MaterialsRice straw with cultivator

MaterialsRice straw with cultivator name of Sazandegi cultivated inZarinshahr (Isfahan, Iran) was used in all experiments. The strawwas milled and screened to size of between 177 and 833 m(20–80 meshes). The enzymes supplied in the experiments werecommercial cellulase (Celluclast 1.5 L, Novozyme, Denmark) and-glucosidase (Novozyme 188, Novozyme, Denmark). The activi-ties of cellulase and -glucosidase were 52 FPU/ml and 240 IU/mlaccording to the method presented by Adney and Baker (1996) andXimenes et al. (1996), respectively. M. hiemalis (CCUG 16148) and S.cerevisiae (CCUG 53310) for ethanolic fermentation were obtainedfrom Culture Collection of Gothenburg University (Gothenburg,Sweden). The fungus was cultivated on agar petri dishes withmedium composition of 40 g/L glucose, 20 g/L agar, and 10 g/L pep-tone at pH 5.5 ± 0.1 and 32 ± 0.5◦C for 5 days. The petri dishes werestored at 4◦C before use (Goshadrou et al., 2011; Karimi et al., 2006).