The furtherchecking of mismatches t

The further
checking of mismatches to all other species either at the 30 position
or where possible, for both forward and reverse primers was performed
by MEGA5 software (Tamura et al., 2011). The designed
primers were also screened for unique specificity to eliminate
cross-species binding with other animal or plant species using
the online BLAST local alignment tool in NCBI data base (http://
blast.ncbi.nlm.nih.gov/Blast.cgi). The designed primers were
purchased from IDT, USA.