These investigations of nucleotide

These investigations of nucleotide biosynthesis and
metabolism in plants have provided a framework for more
detailed analyses of specific enzymes and the isolation of
the corresponding genes. The availability of the complete
genome sequence of Arabidopsis will aid the search for
coding sequences of purine and pyrimidine biosynthesis
and catabolism enzymes based on their sequence similarity
to counterparts identified in other organisms.
Collections of Arabidopsis mutants created by T-DNA
insertions and the development of tilling, a PCR-based
screening procedure to recover chemically-induced
mutants , are valuable resources for
the genetic analysis of these genes.