Accordingly,
CMCase activity was assayed in different reaction buffers
(50 mmol L1 glycine-HCl for pH 3.0; 50 mmol L1 sodium
citrate for pH 3.5e6.0; 50 mmol L1 citrate phosphate for pH 6.5e7.0; 50 mmol L1 phosphate for pH 7.5e8.0; 50 mmol L1
TriseHCl for pH 8.5e10.0) at 50 C in order to determine the pH
profile