1 Principle of the portable and sensitive quantitative detection of DNA
2 Target hybridization with capture DNA on Streptavidin-MNBs
3 Optimization of experimental parameters
Optimization of temperature
Optimization for the dosage of DNA polymerase
Optimization of incubation time for mixture and MBs
Optimization the amount of DNA–invertase conjugation
4 Sensing performance for target DNA
5 Selectivity
6. Feasibility in complex biological matrices
Study of stability for target DNA detection