2.3. PGE2 measurement
RGM1 cells were seeded at 4×105/well in 6-well plates and cultured for 24 h to reach sub-confluence. To silence the Cox- 2 gene expression before plaunotol stimulation, cells were serum-starved for 24 h. Then, the medium was changed and plaunotol was added at different concentrations for up to 6 h. Both the supernatant and the cell lysate were harvested for the