Fig. 1. Mutagenesis scheme. Males w

Fig. 1. Mutagenesis scheme. Males were mutagenized with ENU and crossed to wild-type females four weeks after the treatment. From each individual male
10±20 F1 offspring ®sh were used to establish F2 families by either crossing them to wild type ®sh or by F1 intercrosses. Mutations induced by the treatment are
eventually uncovered in the F3 generation after random intercrosses within the F2 families. F3 siblings of clutches with phenotypes segregating in a Mendelian
fashion were kept for the establishment of mutant lines.