AutoMSMS settings were: MS: 0.5 s (

AutoMSMS settings were: MS: 0.5 s (acquisition of survey spectrum), MS/MS (CID with N2 as collision gas): ion acquisition range: m/z 350–1400, 0.1 s acquisition for precursor intensities above 100,000 counts, for signals of lower intensities down to 1000 counts, acquisition time increased linear to 1.5 s, the collision energy and isolation width settings were automatically calculated using the AutoMSMS fragmentation table; 3 precursor ions, absolute threshold 1000 counts, preferred charge states: 2–4 singly charged ions excluded.