Evaluation of the Effects ofParamet

Evaluation of the Effects of
Parameter Changes
The effects of certain parameters (enzyme type and E : S
ratio) on the ACE-IA of WPC hydrolysates were evaluated.
Four enzymes (proteases from Bacillus licheniformis,
A. oryzae and A. sojae, as well as pancreatin) were used for
preparing hydrolysates with E : S ratios of 0.5:100, 1:100,
2:100, 3:100, 4:100 and 8:100.
Statistical Analysis
All experiments and measurements were performed in triplicate.
A completely randomized factorial design (four
enzymes ¥ six E : S ratios) was adopted to test for significant
effects among the various treatments (P < 0.05). The results
were analyzed with Statistica software (Statsoft 2000).
An analysis of variance was performed to evaluate the
effects of enzyme type and E : S ratio, and the Duncan test
were applied to establish differences between means
(Pimentel-Gomes 2000).
RESULTS AND DISCUSSION
Of the 24 hydrolysates evaluated (Table 2), 11 exhibited very
high ACE-IA (>80%: H1 to H6, H12, H21 to H24) and five
exhibited high activity (>70%: H8 to H11; H20) relative to