HepG2 cells obtained from the Ameri

HepG2 cells obtained from the American Type Culture Collection were maintained in DMEM supplemented with 10% fetal calf serum, 100 U/ml penicillin, 100 lg/ml streptomycin, and 2mML-glutamineandkept at 37 Cina humidifiedatmosphere of 5% CO2. Cells were grown to 70% confluence and then incubated in serum-free medium for 24 h before treatments. To induce FA overloading, HepG2 cells at 70% confluence were exposed to a long-chain oleic acid (OA). OA/BSA complex was prepared as reported previously. Stock solutions of 1 MOA prepared in culturemediumcontaining 1%BSAwere conveniently diluted in culture medium to obtain the desired final concentrations. The OA/BSA complex solution was sterile-filtered through a 0.22 lmpore membrane filter and stored at 20 C.