Protocols for micropropagation of v

Protocols for micropropagation of vanilla have been publishedby several authors using various substances and compositions ofculture media (Kononowicz and Janick, 1984; Phillip and Nainar,1988; Geetha and Shetty, 2000; Giridhar and Ravishankar, 2004;Kalimuthu et al., 2006). However, none of these studies includedtesting the efficiency of the DPS, which has the advantage that theliquid culture medium can be added to each subculture directlyonto the semi-solid medium, thus reducing costs due to greaterspeed in the use of manpower and by using liquid medium in theprocess. To our knowledge, this is the first reported use of thisculture system for shoot proliferation of vanilla.