. Chitosan preparation involves the

. Chitosan preparation involves the deacetlyation of the obtained chitin (Dutta et al. 2004). Deacetylation of chitin involves the removal of acetyl groups from chitin and that was done by employing 70 % NaOH solution with a solid to solvent ratio of 1:14 (w/v) and incubated at room temperature for 72 h. Stirring is mandatory to obtain a homogenous reaction as shown in Fig. 3a–c. The residue
obtained after 72 h was washed with running tap water to neutrality and rinsed with deionized water. It was then filtered, sun dried and finely grinded shown in Fig. 4a. The resultant whitish flakes obtained after grinding are chitosan as shown in Fig. 4b.