ay Body composition analysis ss Body composition was analyzed before and after the to weight loss regime in all dogs, using fan-beam DEXA(Lunar Prodigy Advance; GE Lunar). Pre-weight loss n total body composition results were used to estimate tar get weight[18,21]. Further, by comparing pre- and post weight loss DEXA scan results, change in fat and lean mass could be estimated[12,18]. Definition of metabolic syndrome The guidelines of the International Diabetes Federation[22] were modified in order to produce an accessible system for dogs. Accordingly, we defined ORMD, using e the either the upper limit of the reference range for the laboratory used(e g. cholesterol, triglyceride, and glucose), or based upon internationally accepted criteria defining borderline results(eg. SBP) [23]. Therefore, the final definition was as follows: a) BCS 7-9/9 b) AND any two of the following: 1. Triglycerides >200 mg/dL, (2.3 mmol/L) 2. Total cholesterol 300 mg/dL(7.8 mmol/L) 3. Systolic blood pressure >160 mmHg. 4. Fasting plasma glucose >100 mg/dL(5.6 mmol/L) or previously diagnosed type 2 diabetes mellitus. The prevalence of metabolic syndrome before and after weight loss was assessed based upon these criteria