The association of microbes onto th

The association of microbes onto the rice grain was examined
under scanning electron microscope. The fermented grain was
prepared by fixing with 2.5% para-glutaraldehyde and then dehydrated
by dipping into an ascending gradient of ethanol (20%,
40%, 80%, and 99% for three times). After dehydration, samples
were dried and coated with gold using a Bal-tec SDC 050. The preparations
were observed using a scanning electron microscope
(VEGATESCAN, USA) under 2–12 KX magnification and a constant
acceleration voltage of 10.00 kV.