Second screenhouse experiment From

Second screenhouse experiment
From the first experiment, the two best treatments from the fist screenhouse experiment were selected to evaluate the efficiency of high humus seedling media and beneficial microorganisms on antioxidant activity and vitamin C content.
The two selected media were coconut husk seedling media +10% leonardite +Beijerinckia sp. (VBe 75) and coconut husk seedling media + 15% leonardite +actinomycetes (VAc 077) (Table 1). Selected seedling media (coconut husk compost) was used as control treatment. Randomized complete block design (RCBD) was applied in this experiment (3 treatments with 3 replications) (Table 2). After one week of seedling, each of the two selected isolates was applied to the seedling under each treatment. At the seedling stage and harvest time, content of vitamin C and antioxidant activity were evaluated using the HPLC (High Performance Liquid Chromatography) and DPPH (2,2-diphenyl-1-picrylhydrazyl) method, respectively.