Ferric ion reducing antioxidant power assay (FRAP assay)
Based on table 2, Brazilian mushroom aqueous extract showed significantly higher total antioxidant activity than 60% ethanol extract (p < 0.05). Same pattern also occurred in button mushroom. This showed that aqueous extract had better antioxidant capacities than ethanol extract in both types of mushrooms. Both types of solvent extraction were chosen because the antioxidant capacities of plant samples may be influenced by a lot of factors, such as the extraction solvent and test systems used (Fu et al., 2010). In addition, it is important to use different types of solvent extraction as natural antioxidants are multifunctional. This was shown through the present result reported that button mushroom exhibited significant higher antioxidant activity in aqueous while Brazilian mushroom showed significant higher antioxidant activity in ethanol solvent.