Fig. 2-A shows the electrophoretic

Fig. 2-A shows the electrophoretic profile of total soluble proteins in phosphate buffer. All samples showed the presence of five groups of intense bands. Four of them correspond to polypeptide complexes with molecular weight between 5 and 10 kDa, 20–25 kDa, 34–40 kDa and 50–80 kDa, respectively, and the remaining with greater protein mass (>198 kDa) retained at the top of the separating gel. In general, the major polypeptide band patterns did not differ between the different flour samples. In contrast, some minor polypeptides in the molecular weight range of 10–18 kDa, 28–32 kDa and 96–113 kDa occurred with lower intensities in samples from both HP + P and HP + S treatments (marked with brackets in Fig. 2-A).