The cocoon pieces were added to the solution and boiled without pressure for various lengths of time.
After the extraction, the solutions were filtered through a nonwoven filter to remove the remaining cocoon pieces. The cocoon pieces were then washed several times with warm water, dried in an oven at 50 ◦C for 3 days, and conditioned at room temperature for 12 h before measuring their weights. The degumming ratio was calculated using the following equation: