BBL™ Simmons Citrate Agar
Directions : Suspend 24.2 g of the powder in 1 L of purified water. Mix thoroughly. Heat with frequent agitation and boil for 1 minute to completely dissolve the powder. Autoclave at 121°C for 15 minutes. Cool tube medium in a slanted position for slants. Test samples of the finished product for performance using stable typical control cultures.
Approximate Formula* Per Liter
Ammonium Dihydrogen Phosphate………………….1.0 g
Dipotassium Phosphate………………………………1.0 g
Sodium Chloride……………………………………..5.0 g
Sodium Citrate………………………………………. 2.0 g
Magnesium Sulfate…………………………………...0.2 g
Agar…………………………………………………..15.0 g
Bromthymol Blue…………………………………….. 0.08 g
*Adjusted and/or supplemented as required to meet performance criteria.
For Laboratory Use ▪ Final pH 6.9 ± 0.2
Hygroscopic ▪Keep container tightly closed.
Irritating to eyes,respiratory system and skin.