Pyricularia oryzae Sacc. was Isolat

Pyricularia oryzae Sacc. was Isolated at the Instituto
Agronômico (IAC, Campinas, São Paulo, Brazil) and the
inoculum applied by the spray method (Bonman et al.,
1986). Disease evaluation was also performed according to
Bonman et al. (1986). One very susceptible rice line (line
165) and two resistant lines (line 11 and Labelle) were selected
for molecular analysis. These lines are continuously
maintained in green houses at IAC and checked from time
to time for resistance and susceptibility to P. oryzae.