A 0.1 mL sample of extract (prepare

A 0.1 mL sample of extract (prepared as described in Section 2.6, above, and diluted 2 fold with 80% (v/v) methanol) was added to 3.9 ml of 0.1 mmol/L DPPH (in methanol) then kept in darkness for 30 min at room temperature. Trolox solution was used to construct the cali- bration curves and the results are expressed as TEAC mg/g DW.