Phylogenetic tree revealed the close relationship of the 3 isolates with other rubber degrading bacteria, as shown in Fig. 5. Neighbour Joining tree indicated that, Gordonia sp. CDRG-7 is closely related to G. polyisoprenivorans VH2 (bootstrap ¼ 100). Gordonia sp. CFR-4 was placed within the Gordonia monophyletic clade with lower bootstrap percentage (63 to G. polyisoprenivorans VH2 and 65 to G. westfalica Kb1). Beside, Streptomyces sp. CFMR-7 was placed in a monophyletic taxon with the two Streptomyces strains (bootstrap ¼ 100), indicating its close relationship with Streptomyces sp. K30 and Streptomyces sp. LCIC4.