Aquariums or large plastic boxes ma

Aquariums or large plastic boxes make good arenas for these analyses. Ideally each arena should have a surface area of at least 0.25 m. cover the bottom of each of three containers with a thin layer of finely shredded bark or vermiculite. Place fly pupae on this substrate in a clumped, random, or dispersed pattern, one per arena. The actual number of pupae used will depend on arena size, but must equal for each of the three treatments. A typical 10- gallon aquarium should hold six pupae. For the clumped treatments, use a random number table to choose one corner of the tank and place all six pupae in the corner. For the uniform treatment, place a pupae in each corner and one midway along each side of the tank. For the random treatment, use the dimensions of the tank to form an “X” and a “Y” axis. Use a random number table to obtain six sets of Cartesian coordinates, and place a pupae at each of these points. Replicate each of the three treatments at least five times. Place a single fecund female wasp in each arena and cover with a sheet of glass. After 24 hours, remove all pupae, and place in a labeled vial to incubate at room temperature. Eight to thirteen day later, dissect the pupae and record the number that contained flies or wasps. Alternatively, allow the insects to enclose and record the number of pupal cases that split open and the number that have small exit holes