The variability in PK/PD of resuspe

The variability in PK/PD of resuspended or nonresuspended NPH insulin, reported as CVs in the present study, is not directly comparable with that of another study where NPH insulin was resuspended before injection (7). In that study, the variability of NPH insulin was higher than glargine and detemir in subjects with T1DM. However, the study examined parallel groups of subjects, not the same subjects receiving different basal insulins in a crossover comparison as in the present study. In addition, the clamp technique used (automatic Biostator) (7) is not comparable with that of the current study (manual clamp) (4,14). One limitation of the present study is that calculating within-subject variability for resuspended NPH insulin was not possible because the subjects were studied only once in the R+ condition. Only within-subject variability of resuspended and nonresuspended NPH insulin was calculated (Table 2). As expected, the highest variability occurred between the two extreme situations of injection of the clear and cloudy parts of NPH insulin (R- up and R- down).