2.7. Antioxidant activity determina

2.7. Antioxidant activity determinations 2.7.1. ABTS assays For ABTS assay, the procedure followed the method of Arnao. Cano. and Acosta (2001) with some modifications. The stock solu- tions included 7.4 miM ABTS solution and 2.6 mM potassium per- solution was then prepared by sulfate solution. The working mixing the two stock solutions in equal quantities and allowing them to react for 12 h at room temperature in the dark. This solu- tion was then diluted by mixing 1 mL ABTS solution with 60 mL methanol to obtain an absorbance of 1.1:0.02 units at 734 nm Fresh ABTs solution was prepared using the spectrophotometer. for each assay. Fruit extracts (150 HL) were allowed to react with 2850 of the ABTS solution for 2 h in the dark. Then. the absor The stan using a spectrophotometer. bance was read at 734 dard curve was linear between 25 and 600 mM Trolox. Results are expressed in uM Trolox equivalents (TE)lg dry mass. as means of three replicates Spectrophotometer