Cell cultureThe human lung carcinom

Cell culture
The human lung carcinoma (A549) cell was kindly provided
by the Chulabhorn Research Institute. The human
hepatocellular carcinoma (HepG2) cell (ATCC HB-8065)
was obtained from the National Cancer Institute, Bangkok,
Thailand. The HepG2, and A549 cells were cultured in
RPMI 1640 medium (Hyclone, Logan, UT, USA). Both
cell lines were supplemented with 10% heat inactivated
fetal bovine serum (Hyclone), 100 U/mL penicillin and
100 μg/mL streptomycin (Gibco BRL, Grand Island, NY).
All cell lines were maintained at 37℃ in a 5% CO2 incubator
and the media were changed twice weekly.