Exploration of single cell oil (SCO) production by yeast fermentation began in the 1970s to
produce SCO from microorganisms to substitute lipids from plants as a source of food for
humans and feed for animals [1]. Cheese whey was used to culture oleaginous yeast
Cryptococcus curvatus (Candida curvata) to produce cocoa butter substitute. Fermentation
processes were developed and tested on both pilot scale (0.5-m3 fermentor) and industrial
scale (8.2-m3 fermentor) [2]. Based on these studies, Davies has estimated the