in order to further confirm a conne

in order to further confirm a connection between the COG pro- teins and the mutant phenotypes, each COG gene was down-regu- lated under the alcA promoter. Both strains R785 (alcA(p)::COG2) and G66 (alcA(p)::COG4) exhibited defective polarity establish- ment on alcA-repressing media (1% glucose) at 28 °C (Fig. 7A and C), and wild type polarized growth on alcA-inducing media (1% glycerol) at 28 °C (Fig. 7B and D). When stained with Calcofluor white, the cell wall of these strains grown on 1% glucose displayed staining similar to a mutant strain with swoPCOG4