In the present audit gaseous ozone was used. The choice of gaseous ozone was based on the ozone generator currently available and in use by the authors, it has been approved for use in the EU and room contamination and staff and patient exposure can be significantly reduced by adherence to safety measures including application of suction [18,22]. Attention to safety is important with ozone. In addition, this form of ozone application has not been studied extensively in the periodontal literature. Ozone nano-bubble water has been also studied and shown to have potential as an adjunct to periodontal treatment [10].