The specificity of the each pair of

The specificity of the each pair of the designed primers was checked one by one and also against the fifteen meat-providing and expensive terrestrial (beef, chicken, goat, lamb, buffalo, venison,duck, pigeon, and quail) and aquatic species (salmon, cod,tuna, carp, rohu, and tilapia) and five plant materials (wheat,tomato, garlic, onion, and pepper). In each individual assay, each pair of primers was first tested against other four species of the multiplex system as non-target DNA and then with most commonly used commercially important meat, fish, and plant species and no cross-amplification was detected