For measuring emulsification index,

For measuring emulsification index, hydrocarbons or oils were added to aqueous phase containing the biosurfactant
(0.1%, w/v), in a ratio of 3:2, v/v, followed by vigorous agitation on a cyclo-mixer for 2 min. The oil, emulsion and aqueous layers were measured at every 24 h interval up to 240 h. The emulsification index was noted with respect to time (Cooper & Goldenberg, 1987) and is represented accordingly, i.e. the emulsification indices after 24 h, 48 h, 72 h, etc.