Sample and determination of oil con

Sample and determination of oil content
Two hundred and four Safflower germplasm collections grown in the year 2009 were used for calibration development and validation. Seed samples were oven dried at 380C for 24 h and oil content (%) of intact seed samples determined using a pre-calibrated NMR analyzer (Oxford 4000).The sample size was about 20 g and the NMR integration period was approximately 80 second. Instrument was calibrated to display oil content of the seed sample. Standard safflower oil mixed with glass beads was periodically measured to check the instrument settings.The average of the three readings was recorded for each sample.