The distribution of theelectrostati

The distribution of the
electrostatic potential on the surface of the molecule is non-uniform and identifies one side of the alpha-helical domain
as negatively charged. The predominance of arginine residues
as basic residues ensures that the observed positive charge
distribution is also maintained in the highly alkaline envi-ronment found in the lepidopteran midgut.
The studies on
Cry1Ac toxin revealed that residue 544 of domain III plays an
important role in maintaining structural stability. Substitu-tion of a polar group at this position is unfavorable to its
stability