2.2 Fungal and yeast cultures C. gl

2.2 Fungal and yeast cultures C. gloeosporioides, the fungal pathogen of anthracnose, was isolated from mango, cultivar Nam Dok Mai, showing anthracnose symptom. A pure fungal culture was grown on potato-dextrose agar (PDA) and stored at 5°C until use. The yeast Issatchenkia orientalis was isolated from the ripe sapodilla (named in Thai as La Mud, scientific name: Manilka razapota (L.) P. Royen. Peel and flesh fruit (10 g) cut into small pieces, were shaken in 100 mL of sterile distilled water for 40 min at 150 rpm on a rotary shaker (New Brunswick, Model Imnova 2050, U.S.A.). Aliquots of serial dilutions of the wash were then plated onto PDA. The Petri dishes were incubated at room temperature (30+2°C) for 5 days and the creamy white colonies of the yeast were transferred to fresh PDA plates. The most effective yeast isolate was screened by a dual culture test. Pure culture of the selected yeast isolate was grown on PDA and stored at 5°C until use. Identification of this yeast isolate was carried out by BIOTEC, the National Science and Technology Development Agency and the result revealed it as Issatchenkia orientalis.