A high titre bacteroiphage lysetes

A high titre bacteroiphage lysetes of 10 pfu/mL previously filtered through 0.22 um Minisart Sartorius filter was purified by centrifugation and treated with a fixative 1% glutaraldehyde. A drop of purified and fixed phage lysate was deposited on Formvar carbon-coated copper grids and negatively stained with 1% phosphotungstic acid (PTA). The grids weRE examined in Tecnai G Biotwin transmission electron microscope. The images of phages were captured and measured the size of head and tail.