ABSTRACTMost fixed-time inseminatio

ABSTRACT
Most fixed-time insemination protocols utilize an injection of GnRH at the beginning of the protocol to initiate a new follicular wave. However, the ability of GnRH to initiate a new follicular wave is dependent on the stage of the estrous cycle. We hypothesized that administering PGF2α 3 d before initiating a fixed-time AI protocol would improve synchrony of follicular waves and result in greater pregnancy success. Therefore, our objective was to determine whether inducing luteal regression 3 d before a fixed-time AI protocol would improve control of follicular turnover and pregnancy success to fixed-time AI. Multiparous crossbred cows at 3 locations (n = 108, 296, and 97) were randomly assigned to 1 of 2 treatments: 1) PGF2α [25 mg; intramuscularly (i.m.)] on d −9, GnRH (100 μg; i.m.) and insertion of a controlled internal drug-releasing device (CIDR) on d −6, PGF2α (25 mg; i.m.) and CIDR removal with PGF2α (25 mg; i.m.) at CIDR removal on d 0 (PG-CIDR) or 2) GnRH (100 μg; i.m.) and insertion of a CIDR on d −5 and CIDR removal with PGF2α (25 mg; i.m.) at CIDR removal and 4 to 6 h after CIDR removal (5-d CIDR). Cows were time-inseminated between 66 and 72 h (PG-CIDR) or 70 to 74 h (5-d CIDR) after CIDR removal, and GnRH was administered at the time of fixed-time AI. At location 1, ovulatory response to the first injection of GnRH was determined by ultrasonography at the time of GnRH and 48 h after GnRH administration. Among cows with follicles ≥10 mm in diameter, more (P = 0.03) PG-CIDR-treated cows ovulated after the initial GnRH injection (88%, 43/49) compared with the 5-d CIDR-treated cows (68%, 34/50). Pregnancy outcome was not influenced by location (P = 0.96), age of the animal (P = 1.0), cycling status (P = 0.99), BCS (P = 1.0), or any 2-way interactions (P ≥ 0.13). However, pregnancy success was influenced by synchronization protocol (P = 0.04). Pregnancy outcome was greater (P = 0.04) for the PG-CIDR protocol (64%) compared with the 5-d CIDR protocol (55%). In summary, control of follicular turnover was improved by inducing luteal regression 3 d before initiation of a fixed-time AI protocol, and pregnancy success was improved with the PG-CIDR protocol compared with the 5-d protocol.