ABSTRACT
In vitro raised Acmella calva (DC.) R.K. Jansen was extracted using various solvent systems and screened for their phytochemical and antimicrobial activity using disc diffusion method. Acmella calva was raised through in vitro culture of nodal segments. Murashige and Skoog (MS) medium supplemented with 2.22μM BA was found to be most effective in inducing plantlet growth. The methanol and water extract exhibited good antibacterial and antifungal activity. These finding is important for pharmacology and potential to develop natural active constituents from these improved elite lines would be useful and profitable.
Keywords: Antimicrobial activity, Acmella calva (DC.) R.K. Jansen, Minimum Inhibitory Concentration (MIC), Phytochemical, Murashige and Skoog