The HPTLC Fingerprint of various extract and fraction were developed using CHCl3: ethyl acetate: Formic acid (1:9:0.4) as mobile phase, stationary phase; (pre-coated plate) Silica gel G 60 F254, sample applicator; CAMAG LINOMAT 5, developing chamber; twin trough chamber.
The plate was scanned at 366 nm under fluorescence mode and in visible mode after derivatization with alcoholic FeCl3 using CAMAG TLC SCANNER-3.