A wild genotype (CFRA 110) in F. vi

A wild genotype (CFRA 110) in F. virginiana subsp.
platypetala was found to be a decaploid by chromosome
counting [7]. Our work on the same accession confirmed
the ploidy characterization of CFRA 110 by
showing 70 chromosomes in a mitotic spread. FISH
study detected four 5S and fifteen 25S rDNA sites distributed
over 18 chromosomes, of which one was double-marked by a 25S and a 5S rDNA signal (Figure
3I). In F. iturupensis, the other natural decaploid species
found so far in this genus, six 5S and twelve 25S rDNA
sites were detected over eighteen chromosomes, and no
chromosome was double-marked (Figure 3J). Both decaploids
also presented variable signal sizes and intensities
among 5S rDNA sites.