Results showed that the effects of

Results showed that the effects of temperature and reaction
time on glucose released were statistically significant (p < 0.05)
while the increase in acid concentration from 0.1% to 1.0% (w/v)
was found to be the least significant at the same confidence level.
To determine the optimum conditions for acid pretreatment and
enzyme saccharification of Sargassum spp. an optimization study
was conducted. Statistical analysis also showed that the t-value
of the effect of change in acid concentration from 0.1% to 1.0%
was slightly above the t-value limit which can be interpreted as
possibly significant as opposed to values above the Bonferroni limit
which can be taken with certainty that the effect was significant. In
order to see further the effect of acid concentration on enzyme saccharification,
the acid concentration range used in the optimization
study was changed to 1% as the low level () and 5% as the high
level (+). On the other hand, the levels for the two factors, temperature
and reaction time, remained the same.
The glucose yield after enzyme saccharification of acid treated
biomass is shown in Fig. 1(a). The highest glucose released after
enzyme hydrolysis was obtained for