MATERIALS AND METHODSThe present st

MATERIALS AND METHODS
The present study was performed according to
the norms of ethics and biosafety of the Colegio
de Postgraduados, Campus Montecillo, México.
The coffee (Coffea Arabica) pulp was ensiled
with 5% molasses, fermented for two months, and sun-dried dehydrated in the sun. The coffee
pulp used contained 0.50% caffeine and
antioxidants such as chlorogenic acid (5.61%),
caffeic acid (1.47%) and gallic acid (0.26%).
In order to measure the antioxidant capacity of
the diets, antioxidant extraction was done
according to the technique described by Restrepo
et al. (2009). The antioxidant capacity was then
determined using the FRAP technique by Benzie
and Strain (1999).
Fifteen Blackbelly lambs, with an initial weight
of 22.86±0.60kg and average age of 6 months,
were assigned to one of three treatments:
T0=control diet (n=5), T1=diet with 8% coffee
pulp (n=5), and T2=diet with 16% coffee pulp
(n=5) (Table 1). The experimental design was
completely randomized. After a 12 day period of
adaptation to the diet, the lambs were fed the
treatment diets for 56 days.
At the end of the fattening period the animals
were slaughtered after a 12 hour fasting period
with no water restriction. Average weights were
32.76±0.92, 33.06±0.92, and 33.18±0.92kg for
T0, T1, and T2, respectively. Carcass dressing
was determined by dividing the weight of the hot
carcass by the slaughter weight. Fat in the rumen,
intestines and kidneys was removed manually
and determined as percentage of slaughter
weight. Carcasses were hung and refrigerated for
24 h at 3-4◦C.