Direct amplification fingerprinting

Direct amplification fingerprinting (DAF) is another method based upon amplification of DNA using arbitrary primers. Here the arbitrary primers used are very short (5-8 bases in length). This is not frequently used because this method gives complicated fingerprints consisting of a large number of bands, which could only be distinguished by silver staining technique (not by ethidium bromide/fluorescence). Moreover, separation of these small fragments requires the use of polyacrylamide (instead of agarose) gel.