Results and discussion:Partial Puri

Results and discussion:
Partial Purification of Aloin from plant crud extracts:
The extraction efficiencies of different alcohol / salt ATPSs are shown in Table-1, it can be seen
that 1-propanol/[NH4]2SO4 system has the highest extraction efficiency than other alcohol/salt
systems. To optimize the [NH4]2SO4 concentration, 1.5–3.0 g [18.50–31.23 %, w/w] [NH4]2SO4 was
added into the system. The alcohol/salt system with 17.84 % 1-propanol and 26.66 % [NH4]2SO4 has
the best extraction ability, the extraction efficiency increased with [NH4]2SO4 concentration, it can be
explained that the increase of [NH4]2SO4 will strengthen the salting-out effect and lead to increase the
1-propanol concentration in the alcohol-rich phase. The phase separation of ATPS is widely regarded
as the result of the salting-out effect of salts in the alcohol/salt system [15]. When the salt
concentration reaches saturation, the continuous addition of salt will be precipitated out and the
extraction efficiency will not be increased. Moreover, due to the hydrophobicity of AQs, the
hydrophobic interactions between AQs and alcohol-rich phase probably are the main driving forces for
the extraction
fixative [4°C], and the volume was made up to 5 ml with the fixative. The fixed cell suspension was
incubated in the refrigerator [4°C] for 30 minutes, and after that, it was centrifuged [800 rpm] for 5
minutes. The process of fixation was repeated two times, and by then the cells was suspended in 1 ml
of the fixative. The fixed cells were smeared on a clean slide, and left for air-drying. The slide was
stained with Giemsa stain for 15 minutes, and then rinsed with distilled water, and finally it was airdried.