All fractions obtained from the supernatant of M. aeruginosa PCC 7804, M. aeruginosa Sciento and N.
spumigena were analyzed by HPLC. The chromatograms of standard microcystin LR (retention
time: 24.04 min) and nodularin (retention time: 24.04min) are presented in the Figure 2 and 3. It was found that microcystin LR was present in 25%, 50% and 75% aq. methanol fraction of M. aeruginosa PCC
7804 and 50% aq. methanol fraction of M. aeruginosa Sciento. The 50% aq. methanol fraction of N. spumigena contained nodularin. Comparing the chromatographs with standard microcystin LR and
nodularin it is assumed that 25%, 50% and 75% aq. methanol fraction of M. aeruginosa PCC7804
contained approximately 22.6, 157 and 31.2 ìg/mL toxins, respectively, while 50% aq. methanol fraction
of M. aeruginosa Sciento and 50% aq. Methanol fraction of N. spumigena contained 10 and 28.2
ìg/mL toxins. Other fractions were found to contain an average amount of toxins.
All fractions obtained from the supernatant of M. aeruginosa PCC 7804, M. aeruginosa Sciento and N.spumigena were analyzed by HPLC. The chromatograms of standard microcystin LR (retentiontime: 24.04 min) and nodularin (retention time: 24.04min) are presented in the Figure 2 and 3. It was found that microcystin LR was present in 25%, 50% and 75% aq. methanol fraction of M. aeruginosa PCC7804 and 50% aq. methanol fraction of M. aeruginosa Sciento. The 50% aq. methanol fraction of N. spumigena contained nodularin. Comparing the chromatographs with standard microcystin LR andnodularin it is assumed that 25%, 50% and 75% aq. methanol fraction of M. aeruginosa PCC7804contained approximately 22.6, 157 and 31.2 ìg/mL toxins, respectively, while 50% aq. methanol fractionof M. aeruginosa Sciento and 50% aq. Methanol fraction of N. spumigena contained 10 and 28.2ìg/mL toxins. Other fractions were found to contain an average amount of toxins.
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