A ten-fold dilutionStep 1.Use a mic

A ten-fold dilution

Step 1.

Use a micropipette to dispense 900 mL of the diluent to glass tube.

Step 2.

Use a micropipette to transfer 100 mL of the test solution to the first well. Discard the tip.

Step 3.

Mix by shaking by hand or using a vortex mixer.

Step 4.

The well now contains 100 mL of the original test solution diluted by one tenth in a total volume of 1000 mL.