2.3. Optimization of pretreatment c

2.3. Optimization of pretreatment condition
Response surface methodology is a combination of mathematical
and statistical techniques used for modeling, developing,
improving and optimization of multiple variables, which determine
optimum process conditions by sequential testing procedure
and used to evaluate the relative significance of several affecting
factors even in the presence of complex interactions (Myers and
Montgomery, 2002; Singh and Bishnoi, 2012). In the present study,
central composite design (CCD), with k = 2, was used in order to
50 ◦C for subsequent analysis.
2.2.3. Hydrogen peroxide pretreatment
The substrate at a solid loading of 10% (w/v) was pretreated in
autoclave with hydroxide peroxide at concentrations of 2% (w/v) as
soaking agent for residence time 40 min at 121 ◦C temperature and
15 psi pressure. After cooling, the contents were filtered with two
layers of muslin cloth and residues were washed several times in
tap water to neutralize the pH followed by a final rinse in distilled
water, after that residue was air dried by spreading on paper at
50 ◦C for subsequent analysis.