A preliminary screening to determin

A preliminary screening to determine the amylolytic activity of the
bacteria strains was performed through pour plate technique. Each bacteria strain was inoculated separately at the concentration of 6 log
CFU·mL− 1 in MRS agar (de Man–Rogosa–Sharpe) containing 20 g of
soluble starch. L− 1 (Sigma, ACS reagent 9005-84-9) as the carbon
source. The petri dishes were maintained at 37 °C during 24 h in aerobiosis for L. plantarum A6 and L. fermentum Ogi E1 and during 72 h in anaerobiosis provided by Anaerocult® A (Merck, Darmstadt, Germany) for
the probiotic strains. Afterwards, the colonies were enumerated and
then, the culture plates were covered with some drops of Lugol iodine
and the colonies with halo of starch hydrolysis were counted (Sanni,
Morlon-Guyot, & Guyot, 2002). The results were expressed as % of colonies presenting amylolytic activity